@article {Torro2024.03.15.585250, author = {Remy Torro and Beatriz Diaz Bello and Dalia El Arawi and Lorna Ammer and Patrick Chames and Kheya Sengupta and Laurent Limozin}, title = {Celldetective: an AI-enhanced image analysis tool for unraveling dynamic cell interactions}, elocation-id = {2024.03.15.585250}, year = {2024}, doi = {10.1101/2024.03.15.585250}, publisher = {Cold Spring Harbor Laboratory}, abstract = {A current key challenge in bioimaging is the analysis of multimodal and multidimensional data reporting dynamic interactions between diverse cell populations. We developed Celldetective, a software that integrates AI-based segmentation and tracking algorithms and automated signal analysis into a user-friendly graphical interface. It offers complete interactive visualization, annotation, and training capabilities. We demonstrate it by analyzing original experimental data of spreading immune effector cells as well as antibody-dependent cell cytotoxicity events using multimodal fluorescence microscopy.Competing Interest StatementThe authors have declared no competing interest.}, URL = {https://www.biorxiv.org/content/early/2024/03/17/2024.03.15.585250}, eprint = {https://www.biorxiv.org/content/early/2024/03/17/2024.03.15.585250.full.pdf}, journal = {bioRxiv} }