@article {Awada2026.03.11.710783,
	author = {Lama Awada and Jad Sleiman and Jean-Marc Navarro and Remy Torro and Martine Biarnes-Pelicot and Elodie Hector and Laurent Limozin and Chen Dong and Pierre Milpied and Philippe Robert},
	title = {Antibody maturation in germinal centers selects mutants based on BCR-antigen bond mechanical resistance},
	elocation-id = {2026.03.11.710783},
	year = {2026},
	doi = {10.64898/2026.03.11.710783},
	publisher = {Cold Spring Harbor Laboratory},
	abstract = {Antibody maturation in germinal centers (GCs) is traditionally viewed as a process that enhances antigen-binding affinity of B cell receptors (BCR). However, recent studies challenge this paradigm, revealing no systematic antibody affinity improvement during GC selection. Here, we investigate whether mechanical resistance of antibody-antigen bonds, rather than affinity, is the selective parameter driving GC maturation. Using biolayer interferometry measurements for assessing affinity and laminar flow chamber for mechanical resistance, we analyzed three lineages of mouse antibodies generated after immunization with ovalbumin. While affinity changes were heterogeneous, ranging from gains to losses across lineages, mechanical resistance consistently increased after maturation. Bond lifetimes under physiological forces (10-70 pN) converged to similar values across lineages, suggesting a selective pressure for mechanical stability. Functionally, NK cell activation in a surrogate in vitro ADCC assay correlated with bond lifetimes under force, but not with affinity. This indicates that lymphocytes are sensitive to the mechanical stability of antibody-antigen interactions, which may underlie GC selection. Our findings reconcile antibody maturation with the idea of enhanced binding, while aligning with the role of mechanical forces in B cell signaling and antigen uptake. These insights provide a framework for understanding GC biology and may inform the design of therapeutic antibodies.},
	URL = {https://www.biorxiv.org/content/10.64898/2026.03.11.710783v1},
	eprint = {https://www.biorxiv.org/content/10.64898/2026.03.11.710783v1.full.pdf},
	journal = {bioRxiv}
}