@article{diaz-belloQuantitativeMicroscopyCell2025, title = {Quantitative {Microscopy} for {Cell}–{Surface} and {Cell}–{Cell} {Interactions} in {Immunology}}, volume = {15}, url = {https://bio-protocol.org/en/bpdetail?id=5427&type=0}, doi = {10.21769/BioProtoc.5427}, abstract = {Cell–surface and cell–cell interaction assays are fundamental for studying receptor–ligand interactions and characterizing cellular responses and functions. They play a critical role in diagnostics and in modulating immune system activity for therapeutic applications, notably in cancer immunotherapy. By providing time-lapsed and cell-level direct observation of the sample, optical microscopy offers strong advantages compared to current go-to techniques, which are typically either ensemble methods (e.g., measuring cell populations) or indirect readouts (e.g., impedance for adherent cells). This protocol describes two complementary microscopy-based assays: (1) a cell–surface ligand binding assay to quantify dynamic interactions between human primary Natural Killer (NK) cells and a cancer-mimicking surface, and (2) a cell–cell interaction assay to evaluate antibody-dependent cell cytotoxicity (ADCC) mediated by NK cells targeting tumor cells. Additionally, the protocol uses Celldetective, a new open graphical user interface for quantitative analysis of cell interaction dynamics from 2D time-lapse microscopy datasets. Although applied here to primary immune cells, these methods are adaptable to various cell types, including other immune cells, fibroblasts, and cancer cells. This approach enables direct observation and quantification of cellular morphology, motility, cell–cell interactions, and dynamic behaviors at single-cell resolution over time, facilitating detailed analysis of mechanisms such as cell death, migration, and immune synapse formation.}, language = {en}, number = {17}, urldate = {2025-08-18}, journal = {Bio-protocol}, author = {Díaz-Bello, Beatriz and Arawi, Dalia El and Torro, Rémy and Chames, Patrick and Sengupta, Kheya and Limozin, Laurent}, month = sep, year = {2025}, file = {Snapshot:/home/torro/Zotero/storage/RPJ9YCVG/bpdetail.html:text/html}, }